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KMID : 1161420210240111145
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Journal of Medicinal Food
2021 Volume.24 No. 11 p.1145 ~ p.1152
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Natural Herb Mixture Extract Accelerates Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Activating the SMAD Pathway
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Koh Kwang-Soo
Kim Tae-Young
Kwak Yeon-Ju
Kim Hyung-Joon
Bae Moon-Kyoung
Bae Soo-Kyung
Kim Da-Sol
Jang Hye-Ock
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Abstract
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We aimed to analyze the effects and explore the molecular mechanisms of a natural herb mixture extract (NME) on osteoblasts during differentiation in human bone marrow-derived mesenchymal stem cells (hBMSCs). We tried to confirm the regulation of osteogenic differentiation during NME treatment. Alkaline phosphatase assay and Alizarin red S staining were performed to evaluate the regulation of osteogenic differentiation. Real-time polymerase chain reaction was performed to analyze the expression of osteoblast maker genes, and Western blot was used to verify the signaling pathway. Signaling pathway conformation, selective bone morphogenetic protein receptor inhibitor, and dorsomorphin homolog 1 were used as pretreatments before inducing osteogenic differentiation. We determined that MME (natural herb mixture extract) was a safe material and significantly increased osteoblast differentiation and that SMAD phosphorylation is a key signaling pathway that regulates osteogenic differentiation in hBMSCs.
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KEYWORD
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bone marrow mesenchymal stem cells, bone regeneration, cell signaling, natural-herb mixture extract, regeneration medicine
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